NKp46 Antibody for Flow Cytometry

Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse CD335 (NKp46) Antibody

Catalog Number:

137606

I use this antibody to detect mouse NK cells isolated from blood, spleen, or lung. The antibody works well in identifying the population of interest. The only issue is that the FITC fluorescence is not terribly bright, so NK cells often don't appear as a clearly defined population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood, spleen or lung

Primary Incubation

1:200 30 min at 4C

Blocking Agent

anti-mouse CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytomteter

Results Summary

The antibody works well for detecting NKp46+ NK cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well at relatively low concentration.

The Bad

FITC is not very bright

The Bottom Line

Good NKp46 antibody

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