Tandem Dye Flow Antibody for Mouse CD45

Pathology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Cy7 anti-mouse CD45 Antibody

Catalog Number:

103116

Our lab is interested in understanding the immunological impact of HIV in the context of illicit drug abuse. We utilize several mouse models which require the identification of murine immune cells. The tandem dye antibody allows for the use of the red channel so that other colors can be accommodated in a flow cytometry panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine immune cells

Primary Incubation

APC/Cy7 anti-mouse CD45 Antibody

Blocking Agent

Anti-Fc

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

We see clear distinction of mCD45+ populations in cells isolated from mouse brain. We can also see a clear separation of mCD45 intermediate and mCD45 high populations which allows for better distinction between resident microglia and peripheral leukocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great staining with little to no background

The Bad

None

The Bottom Line

Easy to use and optimize and gives great separation between different immune populations.

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