Good ESET Antibody for Western Blot

September 28, 2018

BlueskyReddit
School of Graduate Studies
SUNY Downstate Medical Center
Graduate Student

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Company:

Cell Signaling Technologies

Product Name:

ESET (D4M8R) XP® Rabbit mAb #93212

Catalog Number:

93212S

The aim was to determine any changes is methylation pattern in mouse prostate cells after knocking down setdb1, using western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1 hour, 1:100 at room temperature

Blocking Agent

5% milk

Secondary Incubation

Goat anti rabbit

Tertiary Incubation

N/A

Detection

Hrp-dab reaction

Results Summary

Setdb1 was clearly detected through western blots howver, changes is methylation pattern by knocking dow Setdb1 were not observed.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Highly specific

The Bad

Degrades fast

The Bottom Line

Good antibody for western blots, highly specific

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