Good Antibody for Mouse IHC-P

September 21, 2018

BlueskyReddit
School of Graduate Studies
SUNY Downstate Medical Center
Graduate Student

Overall

Quality of Results

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Company:

Abcam

Product Name:

Ki67 Antibody

Catalog Number:

ab16667

Our aim is to study cancer development in mouse prostate having various genotypic mutations. Ki67 is a good marker for increased cell growth (proliferation), which is a good biomarker for cancer development.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

mouse paraffin embedded tissues

Primary Incubation

Overnight

Blocking Agent

2% normal goat serum+ 10% BSA in PBS

Secondary Incubation

Goat anti rabbit

Tertiary Incubation

N/A

Detection

Hrp-dab reaction

Results Summary

Ki67 expression was detected specifically in nuclear region of cells. Comparison between controls and mutated mice were done based on number of Ki67 positive cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Highly specific, no cross reactivity

The Bad

Takes overnight

The Bottom Line

Good antibody with high specificity.

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