Homogenizer for Skin Total RNA Extraction

UIC
Research Scientist

Overall

Performance

Ease-of-Optimization

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Company:

OMNI international

Product Name:

Bead Ruptor 12

Catalog Number:

19-050A

The aim is to test if Bead Ruptor 12 could homogenize tough skin tissues well and obtain good quality of RNA for down stream analysis. The results showed it worked excellently.

Experimental Design and Results Summary

Application

Homogenizing skin tissue

Protocol Overview

Speed: 6m/sTime: 45 sCycle: 1-2

Tips

Precool samples on ice before homogenizing and between cycles

Features Summary

No balancing needed.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

It is very easy to use and a powerful tool. No training is needed.

The Bad

Generating heat during homogenizing.

The Bottom Line

We used mouse , rabbit and human skin samples. They all worked well. It is a fantastic homogenizer. Total RNA extracted was good enough for down stream analysis such as microarray or RNA seq. It would have be better if it has a cooling system.

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