New England BIolabs
Hind III
R0104S
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We use this restriction enzyme for cloning. After cloning into a PCR product cloning vector, we digest with this enzyme to get the DNA fragment that will be next clone in the desired vector with the correct frame position.
DNA digestion
DNA
The kit contains the restriction enzyme and the buffer required for the DNA digestion. One hour at 37 degrees is enough for complete digestion.
None
The enzyme digest completely after one hour and provides the fragment digested that can be isolated after running thorugh a agarose gel.
N/A
Good DNA digestion
Good price and good performance