Poor Quality Antibody

October 03, 2018

BlueskyReddit
Pathology
University of Kansas Medical Center
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Rockland Antibodies & Assays

Product Name:

Anti-PIWI-L3 (RABBIT) Antibody

Catalog Number:

600-401-DP5

This study seeks to validate functional genomic mutations observed in human cancer cells resistant to drug therapies. This antibody was used in Western blotting of human cancer cell lysates.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cancer cell lysates

Primary Incubation

1:1000 in 5% BSA in TBS-T, overnight, 4 degrees Celsius

Blocking Agent

5% BSA in TBS-T, 1 hour, RT

Secondary Incubation

1:10,000 (anti-Ms) in 5% BSA in TBS-T, 30 minutes, RT

Tertiary Incubation

N/A

Detection

Chemiluminescence HRP substrate

Results Summary

A number of non-specific bands were observed, but no band was observed at 101 kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

N/A

The Bad

Did not detect a band at 101 kDa, difficult to know identity of the non-specific bands.

The Bottom Line

Would not recommend purchasing this antibody to detect PIWI-L3.

Share your experience with other scientists. Write a Review! »

Join the discussion