Does Not Recognize Protein at Correct Size

Pathology
University of Kansas Medical Center
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Bio-Rad

Product Name:

SATB1 antibody, clone OTI13D6

Catalog Number:

VMA00155

This project seeks to validate putative functional mutations identified in human cancer cell line models of drug resistance. This antibody was used for Western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cancer cell lysates

Primary Incubation

1:1000 in 5% non-fat milk in TBS-T, overnight, 4 degrees Celsius

Blocking Agent

5% non-fat milk in TBS-T, 1 hour, RT

Secondary Incubation

1:10,000 (anti-Ms) in 5% non-fat milk in TBS-T, 30 minutes, RT

Tertiary Incubation

N/A

Detection

Chemiluminescence substrate

Results Summary

A single band at approximately 40kDa was observed on the film. No band was observed at the expected size of 100 kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

N/A

The Bad

The antibody did not recognize a protein at the predicted size

The Bottom Line

Would not recommend using this antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion