Assay System for Quantifying Exosomes

University of Montana
DBS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

System Biosciences

Product Name:

Exocet Assay System

Catalog Number:

EXOCET96A-1

We are interested in effects of aryl hydrocarbon receptor activation in dendritic cells. This kit was used to quantify exosomes following purification protocols.

Experimental Design and Results Summary

Application

Exosome quantification

Starting Material

Purified exosomes from murine plasma and cell culture supernatants

Protocol Overview

The protocol has you lyse exosomes, centrifuge to remove debris, and load contents into kit. The kit provides necessary reagents and protocols.

Tips

None

Results Summary

This kit gave variable results that did not appear to align with protein concentrations.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Fast

The Bad

Expensive for limited assays

The Bottom Line

Unfortunately the reagents go quickly with a couple trouble shooting runs, which has made it an expensive way to quantify exosomes. The protocol suggests running a calibration set first and this uses quite of bit of the kit's reagents.

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