CD186 (CXCR6) Antibody for PBMCs

September 20, 2018

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

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Company:

Biolegend

Product Name:

PE/Cy7 anti-human CD186 (CXCR6) Antibody

Catalog Number:

356012

This anti-CXCR6 (CD186) antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes (from human PBMCs). We are particularly interested in the innate compartment of lymphocytes. CXCR6 is a chemokine receptor, also known as CD186, is used as a marker for activated and memory T lymphocytes in this panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:50, 37°c for 15min

Blocking Agent

PBS/BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

Antibody identified the CXCR6+ cells within PBMCs, however it is not exceedingly bright. No absolute separation of negative and positive populations was obtained at any titration.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Identified CXCR6pos cells

The Bad

Resolution not the highest

The Bottom Line

Alright

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