Human Anti-CD8-APC Antibody

September 17, 2018

BlueskyReddit
Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Miltenyi

Product Name:

Anti-CD8 APC human

Catalog Number:

130-110-679

This anti-CD8 antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes (from human PBMCs). Clone REA734 recognizes the human CD8 antigen, a cell-surface glycoprotein also known as Leu-2 or CD8a. We are particularly interested in the innate compartment of lymphocytes and we use CD8 to target, and also exclude, a particular subset of T lymphocytes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:100, 37°c for 15min

Blocking Agent

PBS/BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

This antibody stained CD8 expressing cells cleanly and brightly on PBMCs

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable, specific, bright

The Bad

N/A

The Bottom Line

Clean, clear and bright antibody to stain CD8.

Share your experience with other scientists. Write a Review! »

Join the discussion