Anti-Human CD19 BV605 Antibody

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 605™ anti-human CD19 Antibody

Catalog Number:

302243

This anti-CD19 antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes. We are particularly interested in the innate compartment of lymphocytes, and use bright CD20 and CD19 recognizing antibodies to identify and exclude B lymphocytes. CD19 BV605 used at 1:50 dilution, on freshly isolated human PBMCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:50, 37°c for 15min

Blocking Agent

PBS/BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Clear population of CD19+ cells in PBMCs, the antibody is reliable.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable, specific, bright

The Bad

N/A

The Bottom Line

Good antibody to detect CD19+ B cells.

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