Good Option for CD43 Staining in Mouse

September 12, 2018

BlueskyReddit
B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Pharmingen

Product Name:

anti-mouse CD43 FITC

Catalog Number:

553270

My research aims at the elucidation of the roles of B cells and plasma cells in health and disease. In this experiment, I aimed at analyzing the expression of CD43 in B cells and CD4 T cells were used as control.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

anti-CD43FITC at 1/100, 4C, 15min

Blocking Agent

Fc Block (clone 2.4G2), 4C, 15min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

In spleen, CD4 T cells express CD43 while B cells do not express it.

DOI or PMID #

30005826

Additional Notes

These antibody is also good enough to identify development fractions pf B cells in the BM.

Related Categories

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Summary

The Good

Works well

The Bad

FITC is a very common dye.

The Bottom Line

Good option

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