Good Kit for Cloning Screening

CIBER BBN
Pharmaceutical Technology
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Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

QIAprep Spin Miniprep Kit

Catalog Number:

27104

We use this kit for extracting small amounts of plasmids from different Escherichia coli colonies to confirm the presence of a clone inside the colony. It is really easy and quick for DNA plasmid extraction.

Experimental Design and Results Summary

Application

Plasmid DNA extraction

Starting Material

Escherichia coli containing the plasmids after transfection

Protocol Overview

It is really easy to follow the instructions provided my the manufacturer. If they are followed as described, good extractions are obtained

Tips

Follow carefully manufacturer recommendations

Results Summary

We obtain small amounts from each colony studied, with enough amount of DNA plasmid to confirm the presence of a detemined clone.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to perform the extraction

The Bad

none

The Bottom Line

It is really easy and quick for DNA plasmid extraction

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