Biolegend
Anti-mouse CD71 Pe-CY7
113811
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Our lab is interested in studying the T cells that are involved in the clearance of mouse influenza virus. We are curious about their metabolic changes while clearing the infection so we used the marker CD71. We chose this antibody because it’s very bright and should allow us to see any changes that may occur.
Flow Cytometry
Mouse cells
Cells were stained in 100uL FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
none
LSR II
This antibody was bright enough to clearly stain the CD71+ population from our sample.
N/A
Stable and bright
Great antibody to show small shifts in CD71 expression in the mouse.