Great Antibody for Mouse CD5

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Anti-mouse CD5 Alexa Fluor 488

Catalog Number:

100612

Our lab is interested in studying the T cells that are involved in the clearance of mouse influenza virus. CD5 is expressed on T cells and we are interested to see if that expression changes at all in different mouse populations or under different experimental settings. This antibody is nice and bright so we can more easily see any potential changes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse cells

Primary Incubation

Cells were stained in 100uL FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

none

Tertiary Incubation

none

Detection

LSR II

Results Summary

Using this antibody we can easily measure any CD5+ expression on T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stable and bright

The Bad

none

The Bottom Line

This antibody is bright enough to see small expression changes of CD5.

Share your experience with other scientists. Write a Review! »

Join the discussion