Biolegend
Anti-mouse Ly6c BV711
128037
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Browse Similar Products: Ly-6C Flow Cytometry Antibodies
Our lab is interested in studying the T cells that are involved in the clearance of mouse influenza virus. Ly6c is known to be expressed on T cells and be a marker of TH1 differentiation. We are interested to see if this expression changes on T cells based on different mouse types or treatment models. We chose BV711 because it’s very bright and should allow us to see any subtle changes.
Flow Cytometry
Mouse cells
Cells were stained in 100uL FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
none
LSR II
We used this antibody and were able to identify Ly6c expressing cells in our sample.
N/A
Stable and bright
This antibody is very bright and stable to use in our mouse samples.