Great KLRG1 Mouse Antibody

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse KLRG1 APC

Catalog Number:

138414

Our lab is interested in studying the T cells that are involved in the clearance of mouse influenza virus. Specifically we are looking at the effector functions of those T cells and how different treatments and experimental settings change the effector expression of markers such as KLRG1. This antibody is stable and bright enough for our use.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse cells

Primary Incubation

Cells were stained in 100uL FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

none

Tertiary Incubation

none

Detection

LSR II

Results Summary

This antibody is the right brightness to identify any changes in KLRG1 expression in our cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Stable and bright

The Bad

none

The Bottom Line

This antibody is bright to capture small populations without spillover.

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