CD8 Antibody That Works Well

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse CD8 BV650

Catalog Number:

100741

Our lab studies the roll of T cells in the clearance of influenza virus and memory functions. To do any analysis of these T cells, we need a reliable CD8 T cell marker that clearly defines this population. We chose this antibody because it’s bright without extra background stain.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse cells

Primary Incubation

Cells were stained in 100uL FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

none

Tertiary Incubation

none

Detection

LSR II

Results Summary

Using this antibody we can clearly isolate the CD8 T cell population in our mouse cell sample.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stable and bright

The Bad

none

The Bottom Line

This is a reliable antibody for staining CD8 cells in mouse.

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