Invitrogen
CD235a Monoclonal Antibody (CLB-ery-1 (AME-1)), FITC
MHGLA01
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The aim of our research is to detect paroxysmal nocturnal haemoglobinuria (PNH) on red blood cells (RBC). We needed a good antibody that stains the RBCs to distinguish them from debris and other small cells in peripheral blood. We chose this antibody because it was recommended to us by the clinical lab at UK.
Flow Cytometry
Whole blood
5uL antibody [15 minutes at room temp (in the dark)]
None
Detected on the FITC (FL-1) channel BP530/30
The results show very good staining of the RBCs with the FITC-conjugated antibody. As shown in the image, we used FSC/SSC in log to gate on the RBCs (gate: P1) and then check their staining with CD235a using a histogram. Positive cells are shown in gate P2.
N/A
It may take some time to titrate
Strong staining
May take a while to titrate it to the optimal concentration
A good antibody, which identifies RBCs clearly. The fact that it's ASR is an added bonus!