Santa Cruz Biotechnology
Calnexin Antibody (AF18)
sc-23954
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: Calnexin Immunofluorescence Antibodies
My experiment was focused on evaluating the effect of a variant on the localisation of a secreted protein. My hypothesis was that the variant could impair the physiologic protein secretion. I used the anti-calnexin antibody as a marker of ER, to localise the wild-type and mutant protein along the secretory pathway. I used the Calnexin Antibody (AF18) for immunofluorescence on HEK293 cells.
Immunofluorescence
HEK293 cell line
Antibody diluted 1:75 in blocking solution, incubated for 2 hrs at RT
BSA 2%
Dylight 549 goat anti-mouse secondary antibody (115-505-174) diluted 1:200 in blocking solution 1 hr at RT
N/A
Olympus FluoView FV1000
The antibody works well in immunofluorescence on paraformaldehyde fixed cells.
Specific stain
At higher dilution, the signal is lower
This antibody is OK for immunofluorescence on PFA fixed cells.