Santa Cruz Biotechnology
OctA-Probe Antibody (H-5)
sc-166355
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My experiment was focused on evaluating the effect of a variant on the localisation of a cytoplasmic protein. I overexpressed wild-type and mutant isoforms of the protein tagged with FLAG and studied their localisation. The OctA-Probe antibody was used for immunofluorescence on HEK293 cells transiently transfected with a FLAG-fused protein.
Immunofluorescence
HEK293 cell line
Antibody diluted 1:100 in blocking solution, incubated for 2 hrs at RT
BSA 2%
goat anti-mouse AlexaFluor 555 (A28180) diluted 1:200 in blocking solution 1 hr at RT
N/A
Olympus FluoView FV1000
The antibody performs well in immunofluorescence on paraformaldehyde fixed cells. Low background as compared to stain performed on non transfected cells. It also works with a 1:200 dilution.
Specific stain and bright signal also at higher dilutions
Nothing at the moment
I only tested the antibody in immunofluorescence and it is specific and easy to optimise.