Usable Anti-Mouse IgG1

September 07, 2018

BlueskyReddit
B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Ease-of-Optimization

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Company:

Invitrogen

Product Name:

AlexaFluor647 goat anti-mouse IgG1

Catalog Number:

A21240

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) in health and disease. This experiment aimed at measuring the amount of intracellular IgG1 expressed by CD138hi cells at day 5 after immunization with NP-LPS.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Surface staining with CD138 BV421, 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

After permeabilization with cytofix/cytoperm anti-IgG3 FTIC 1/200000 in Perm wash buffer, 20 min 4 C

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Around 1.5% of the CD138hi cells are IgG1 at day 5 after immunization with NP-LPS.

DOI or PMID #

N/A

Additional Notes

I used another anti-mouse IgG1 and obtain the same %.

Image Gallery

Summary

The Good

It will last forever

The Bad

Too much background

The Bottom Line

You can use it, but is not the best option

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