Good TIMP-1 Antibody for Western Blotting

August 29, 2018

Aging
Buck Institute
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Boster Bio

Product Name:

TIMP-1 antibody

Catalog Number:

PA1385

We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. In summary, this antibody is good for human samples and I recommend it.

Experimental Design and Results Summary

Applications

Western Blot

Sample

human NSC cell lysates

Primary Incubation

1:250, overnight at 4 degree C

Blocking Agent

5% milk in TBST

Secondary Incubation

1:2000, 2h at RT

Tertiary Incubation

NA

Detection

ECL chemiluminescence (Pierce kit)

Results Summary

Detects a band at 28kD..... predicted molecular weight of TIMP-1

DOI or PMID #

10.3389/fnins.2017.00736

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody for TIMP-1, cleaner than other antibodies I have tested

The Bad

Detects additional bands above 60 kD..... but this could be due to potential interactions of TIMP-1 with other proteins

The Bottom Line

Good for human samples...... highly recommend this antibody

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