Good CD86 Antibody

September 06, 2018

BlueskyReddit
Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Rat Anti-Mouse CD86 from BD Biosciences

Catalog Number:

553691

In this project the phenotyping of immune cells in murine skin and lymph node was investigated. The immune cells were attracted and activated by injected extracellular vesicles from mature murine dendritic cells. CD86 was one of many markers in our analysis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine spleen

Primary Incubation

1:10; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives good signals on frozen-tissue sections, butbackground is present using this antibody.

DOI or PMID #

none

Additional Notes

none

Related Categories

Image Gallery

Summary

The Good

Shows specific staining

The Bad

Some background staining

The Bottom Line

All in all a good antibody

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