Great CD8a Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Rat Anti-Mouse CD8a from BD Biosciences

Catalog Number:

553031

In this project the phenotyping of immune cells in murine skin and lymph node was investigated. The immune cells were attracted and activated by injected extracellular vesicles from mature murine dendritic cells. CD8a was one of many markers in our analysis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine lymph node

Primary Incubation

1:10; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives very specific and signals on frozen-tissue sections with no background. Publishable results!

DOI or PMID #

none

Additional Notes

none

Related Categories

Image Gallery

Summary

The Good

Specific signals, no background, easy to use!

The Bad

none

The Bottom Line

Will buy and use it again. Recommendable!

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