Very Good Nestin Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Nestin Antibody from BioLegend

Catalog Number:

656812

In this project the pathogensis of melanoma was investigated. With the MELC technology, we analyzed protein expression profiles in nevi and melanomas. Here we focused on the nestin expression in the basal layer of the epidermis. This Nestin antibody gives very specific signals.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (melanoma)

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals (Alexa488) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives very specific signals on frozen-tissue sections with little background. Publishable results!

DOI or PMID #

none

Additional Notes

none

Related Categories

Image Gallery

Summary

The Good

Specific signals, easy to use!

The Bad

none

The Bottom Line

Will buy and use it again. Recommendable!

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