NFAT Antibody in a Dot Blot

Our laboratory studies T Cell Acute Lymphoblastic Leukemia (T-ALL), a cancer of developing T cells. In order to examine the composition and organization of the tumor microenvironment, our lab utilizes a wide variety of markers to identify specific cellular subsets of the tumor microenvironment. We picked this antibody for immunofluorescence imaging knowing that it would characterize the architecture of the tumor microenvironment in T-ALL in our mouse model. NFAT is a key transcription factor in T cell activation that translocates from the cytoplasm into the nucleus following T cell activation. In my experiment, I used a dot blot to confirm NFAT translocation in Jurkat cells.