Biolegend
PE/Cy7 anti-human/mouse/rat CD278 (ICOS) Antibody
313520
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Our goal analyze the response different cell types in mouse lungs after pulmonary bacteria infection. Mouse lungs were digested with collagenase, strained, and stained for flow cytometry with a cocktail of different antibodies. The samples were analyzed on a BD LSRII flow cytometry. The FACS plot shown are after gating on Single, Live, CD45+, CD19+ Cells
Flow Cytometry
Collagenase-digested mouse lung tissue
1:200 for 20 minutes on ice
24G2 in 3% BSA in PBS for 10 minutes on ice prior to staining in 3% BSA
None
Flow cytometer (BD LSRII)
This antibody stained specifically, and a + vs - population was detectable. However, it is notable that this staining appears as more of a gradient than a isolated population. A good FMO or isotype control is useful for this antibody.
N/A
Specific and able to separate + and - populations
Gradiant, but more due to marker than the antibody
Would recommend this antibody and fluorophore choice for ICOS