OPD for Peroxidase Detection in ELISAs

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

SIGMAFAST™ OPD

Catalog Number:

P9187-50SET

We perform a lot of ELISA assays to detect antibodies to HIV from different samples. At the final step we utilize the OPD reagent to detect the HRP-conjugated secondary antibodies. This product is always reliable.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Different samples that have been detected via HRP-conjugated secondary antibodies

Protocol Overview

Very straightforward - prepare the the solution in 20 ml with each tabled as indicated in instructions

Tips

Prepare it as close as possible to time needed. Protect from light

Results Summary

Works very well

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Reliable

The Bad

None

The Bottom Line

I highly recommend it.

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