Anti-Mouse CD49a for Labeling iNKT Cells

September 06, 2018

BlueskyReddit
Developmental Immunology
La Jolla Institute for Allergy and Immunology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

BV711 Hamster Anti-Rat/Mouse CD49a Clone Ha31/8

Catalog Number:

564863

The general focus of my work is to analyze the response different cell types in mouse lungs after pulmonary bacteria infection. We used this antibody to label a subset of iNKT cells from mouse lung tissue.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Collagenase-digested mouse lung tissue

Primary Incubation

1:200 for 20 minutes on ice

Blocking Agent

24G2 in 3% BSA in PBS for 10 minutes on ice prior to staining in 3% BSA

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer (BD LSRII)

Results Summary

This antibody gives specific staining of CD49a on iNKT cells. The population of CD49a+ cells is bright and clearly separated. We tested several different fluorophores, and consistently use BV711 for this marker.

DOI or PMID #

N/A

Additional Notes

Gated on live tetramer+ cells

Related Categories

Image Gallery

Summary

The Good

Clear, specific CD49a+ population

The Bad

None

The Bottom Line

Great antibody, enables clear population detection

Share your experience with other scientists. Write a Review! »

Join the discussion