CD45.1 Antibody for Immunofluorescence Imaging

Molecular Biosciences
University of Texas Austin
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse CD45.1 (Clone A20) Antibody

Catalog Number:

110705

Our laboratory studies T Cell Acute Lymphoblastic Leukemia (T-ALL), a cancer of developing T cells. In order to examine the composition and organization of the tumor microenvironment, our lab utilizes a wide variety of markers to identify specific cellular subsets of the tumor microenvironment. We picked this antibody for immunofluorescence imaging knowing that it would characterize the architecture of the tumor microenvironment in T-ALL in our mouse model. I used this CD45.1 antibody to identify immune infiltrates in a fresh frozen tissue of a cancer tumor model with immunoflourescence imaging.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Thymus

Primary Incubation

1:200 room temp for 1 hour

Blocking Agent

TNB blocking buffer, perkin elmer

Secondary Incubation

None

Tertiary Incubation

None

Detection

DMi8 Leica Microscope

Results Summary

This antibody works great! There are absolutely no problems to report. I've also tried it with the AF 594 and APC conjugates with no issues!

DOI or PMID #

Not yet published

Additional Notes

N/A

Related Categories

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Summary

The Good

This works great on all of the lymphoid organs that I tried when I was doing immunoflourescence imaging!

The Bad

I can't think of any issues I ever had with this antibody.

The Bottom Line

Amazing results! I can't think of a reason to not recommend

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