Dako / Agilent
Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein
Z0334
Browse Similar Products: Glial Fibrillary Acidic Protein Immunofluorescence Antibodies
We've used this antibody to stain astrocytes in organotypic brain cultures to check the possible toxic effects of bilirubin.We selected this antibody because GFAP is a well-known marker of astrocytes and we were interested in an antibody with specificity to obtain a clean image.
Immunofluorescence
Rats organotypic brain cultures from different brain regions
4.8 ug/mL of antibody for 2 hours at 37°C in blocking solution
Blocking solution: 1% normal goat serum + 0.1% triton-X100 in PBS
FITC-Conjugated antibody diluted 1:2000 for 2 hours at room temperature in blocking solution
none
Fluorescent microscopy Leica DM2000
The antibody allows a clear staining of astrocytes with a strong signal. Easy to optimize, without any problems.
N/A
Easy to optimize
None
A good quality antibody, with an high specificity.