Biolegend
Alexa Fluor® 700 anti-mouse Ly-6G Antibody (Clone 1A8)
127622
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The general focus of my work is to analyze the response different cell types in mouse lungs after pulmonary bacteria infection. This antibody was used to specifically stain neutrophils from mouse lungs for flow cytometry.
Flow Cytometry
Collagenase-digested mouse lung tissue, including neutrophils
1:200 for 20 minutes on ice
24G2 in 3% BSA in PBS for 10 minutes on ice prior to staining in 3% BSA
None
Flow cytometer (BD LSRII)
AF700 is a dim marker, but due to the high expression of surface Ly6G this antibody is adequate for good separation of Ly6G+ neutrophils.
N/A
Bright staining, specific for neutrophils
Clear, specific staining. Good choice for the relatively dim AF700 fluorophore.