Prox-1 Antibody for Lymphatic Endothelial Cells

August 17, 2018

PRIMATE RESEARCH CENTER
UNIVERSITY OF WISCONSIN
Scientist I

Overall

Quality of Results

Ease-of-Optimization

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Company:

Reliatech

Product Name:

Human Prox-1 antibody

Catalog Number:

102-PA32AG

Browse Similar Products: Prox-1 Immunofluorescence Antibodies

This antibody was used to detect lymphatic endothelial cells derived from human embryonic stem cells by differentiating in an OP9 co-culture. Prox-1 is a important marker for lymphatic endothelial cells and hence this antibody was selected.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Lymphatic endothelial cells derived from human enbryonic stem cells

Primary Incubation

1:500, over night under refrigeration

Blocking Agent

Rabbit serum in PBST

Secondary Incubation

1:2000, 1hr at room temp, mouse anti-rabbit secondary antibody

Tertiary Incubation

none

Detection

Fluorescence

Results Summary

The antibody works very well in detecting Prox-1 expressing lymphatic endothelial cells.

DOI or PMID #

29583085

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very easy to use and optimize

The Bad

none

The Bottom Line

A good antibody for detecting lymphatic endothelial cells

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