Perfect CD71 Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Mouse Anti-Human CD71

Catalog Number:

555536

In this project the pathogensis of melanoma was investigated. With the MELC technology, we analyzed protein expression profiles in nevi and melanomas. This antibody was used to study the CD71-expression in human skin tissue (melanoma) by direct immunfluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (melanoma)

Primary Incubation

1:40; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives very specific and strong signals on frozen-tissue sections with no background. Publishable results!

DOI or PMID #

none

Additional Notes

none

Related Categories

Image Gallery

Summary

The Good

Specific signals, no background, easy to use!

The Bad

none

The Bottom Line

Will buy and use it again. Recommendable!

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