Excellent MCSP Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 488 Mouse Anti-Chondroitin Sulfate

Catalog Number:

562413

In this project the pathogensis of melanoma was investigated. With the MELC technology, we analyzed protein expression profiles in nevi and melanomas. This antibody was used to characterize melanoma cells in human skin tissue (melanoma) by direct immunfluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (melanoma)

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals (Alexa 488) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signals on frozen-tissue sections with almost no background. Publishable results!

DOI or PMID #

none

Additional Notes

none

Related Categories

Image Gallery

Summary

The Good

Specific signals

The Bad

none

The Bottom Line

Will buy and use it again. Recommendable!

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