Good CD206 Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD206 (MMR) Antibody from BioLegend

Catalog Number:

141704

In this project the phenotyping of immune cells in murine skin and lymph node was investigated. The immune cells were attracted and activated by injected extracellular vesicles from mature murine dendritic cells. This antibody was used to study the CD206-expression in murine skin tissue by direct immunfluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine skin

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signals on frozen-tissue sections, but expect some background staining.

DOI or PMID #

none

Additional Notes

none

Related Categories

Image Gallery

Summary

The Good

Good signals

The Bad

Background staining

The Bottom Line

Overall it is a good antibody

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