A Really Consistent And Specific Microglial Marker

July 30, 2018

Pharmacology and Physiology
Georgetown University Medical Center
PhD Candidate

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Company:

Biolegend

Product Name:

Purified anti-P2RY12 Antibody

Catalog Number:

848002 (clone S16007D)

We applied Biolegend's purified anti-P2RY12 antibody to vibratome-sliced fixed mouse brain tissues. Comparison of the immunofluorescent staining with signal from CX3CR1-GFP mice further confirmed coverage and specificity.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Brain slices

Primary Incubation

5ug/ml, overnight, 4C

Blocking Agent

10% NGS and 1%BSA in 0.1%PBS-Tween20

Secondary Incubation

2.5 ug/ml goat anti-rat AF 647 (Biolegend #405416)

Tertiary Incubation

N/A

Detection

Confocal laser scanning microscopy, 60x objective

Results Summary

This Ab yielded highly specific and intense signal in microglia in vibratome-sliced fixed brain tissue in mice. The microglial specificity of the Ab staining was further confirmed by co-localization with CX3CR1-GFP signal. Furthermore, at all slice thicknesses tested, Ab penetration was not a limiting factor to depth of imaging.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Fantastic marker for microglia in mouse

The Bad

Purified Ab is more expensive, and may not be worth it for some assays.

The Bottom Line

This Ab gave us great immunofluorescence results, with low background and high quality staining of even the most minute microglial processes.

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