Great Anti-Mouse IgG for Western Blot

July 18, 2018

Neurosurgery
University of Pittsburgh

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Licor

Product Name:

IRDye® 680RD Goat anti-Mouse IgG (H + L)

Catalog Number:

925-68070

The secondary antibody for western blotting from LiCor are very good in quality. They are cost-effective, easy to use, simple but sophisticated and reliable for our work. I am using this antibody for last 7/8 years and published lot of papers using them. I will highly recommend this antibody for western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat and mouse tissue samples

Primary Incubation

Overnight at 1:500 at 4 degree centrigrade

Blocking Agent

5% BSA

Secondary Incubation

1 hour at 1:5000 at room temperature

Tertiary Incubation

N/A

Detection

Odyssey® CLx Imaging System

Results Summary

The result using this secondary antibody from LiCor are very good in quality. I am using this antibody for last 7/8 years and published lot of papers using them. I will highly recommend this antibody for western blotting.

DOI or PMID #

https://doi.org/10.1016/j.physbeh.2017.02.027

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy, cost-effective, high quality and reliable

The Bad

some times background is more than conventional method

The Bottom Line

The secondary antibody for western blotting from LiCor are very good in quality. They are cost-effective, easy to use, simple but sophisticated and reliable for our work. I am using this antibody for last 7/8 years and published lot of papers using them. I will highly recommend this antibody for western blotting.

Share your experience with other scientists. Write a Review! »

Join the discussion