CD16/32 Fc Blocking Antibody

Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified anti-mouse CD16/32 Antibody

Catalog Number:

101302

We use this antibody to block non-specific Fc receptor binding in many flow cytometry assays. It works well and is very inexpensive. We almost never have any unusual non-specific staining in our cells when we use this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood, lung, spleen, bone marrow, etc.

Primary Incubation

1:25 dilution, 5 min at 4C before incubating with other antibodies

Blocking Agent

Anti-mouse CD16/CD32

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer

Results Summary

Works very well at blocking non-specific binding.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works well

The Bad

None

The Bottom Line

Very good Fc block, and inexpensive.

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