Average Rb Antibody

July 10, 2018

Pathology
University of Kansas Medical Center
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Rb (4H1)

Catalog Number:

9309

We are interested in mechanisms of resistance to targeted therapies in ovarian cancer. This experiment was designed to determine differential Rb1 protein expression and post-translational modifications in ovarian cancer cells lines resistant to a therapy, compared with therapy-sensitive controls.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human ovarian cancer cell line lysates

Primary Incubation

1:1000 dilution in 5% BSA in TBS-T, overnight at 4 degrees C

Blocking Agent

5% BSA in TBS-T

Secondary Incubation

1:10,000 in 5% BSA in TBS-T, 30 minutes at RT

Tertiary Incubation

N/A

Detection

Chemiluminescence substrate for HRP-labeled secondary antibodies

Results Summary

Observed a strong band at the correct predicted size for Rb (106 kDa), along with additional bands. The larger bands may be posttranslationally modified proteins. The smaller bands are likely non-specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice, clear bands at correct predicted size

The Bad

Non-specific bands, but they don't interefere with interpretation of standard Western blot

The Bottom Line

Average antibody overall, there may be other options that are more well suited for use in Western blots.

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