Anti-Mouse CD45 Antibody

June 15, 2018

BlueskyReddit
Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Pacific Blue anti-mouse CD45 Antibody

Catalog Number:

103125

This antibody is used a lot in our lab in flow cytometry panels to detect various lymphocyte populations, primarily NK cells. The CD45 antibody yields good results in a wide range of panels, and in various fix/perm protocols.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood, spleen, lung, bone marrow

Primary Incubation

1:200 dilution, 30 min at 4C

Blocking Agent

Fc block (Anti CD16/CD32)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer

Results Summary

Stains all lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good reliable staining

The Bad

None

The Bottom Line

Excellent antibody

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