Consistent Staining

Neuroscience
Thomas Jefferson University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

MilliporeSigma

Product Name:

Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4

Catalog Number:

MAB397

To understand how conditioned media from astrocytes expressing mutant ALS linked FUS protein regulate AMPA receptors in motor neurons

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rat motor neurons

Primary Incubation

1:200

Blocking Agent

Horse serum with BSA

Secondary Incubation

1:500 Alexa fluor

Tertiary Incubation

N/A

Detection

Confocal

Results Summary

GluR2 MAB397 antibody provides excellent staining along the dendrites and in the cell soma of motor neurons. Using this antibody we could elucidate reduction in GluR2 levels of motor neurons treated with mutant FUS ACM

DOI or PMID #

29380416

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Consistent staining pattern and monoclonal in nature

The Bad

None

The Bottom Line

Easy to optimize and use AMPA receptor antibody

Share your experience with other scientists. Write a Review! »

Join the discussion