UGDH Antibody for Western Blot

July 16, 2018

Institute of Bioinformatics and Structural Biology
National Tsiing Hua University
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

UGDH antibody

Catalog Number:

GTX104993

In my research, I want to elucidate the metastasis mechanism in ovarian cancer. I used this antibody for western blot to monitor protein expression level in cancer cell lines. Because of the good specificity, we used this product for our experiment.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Ovarian cancer cell lysate

Primary Incubation

1:2000 in TBST, overnight, 4℃

Blocking Agent

Skimmed milk in TBST

Secondary Incubation

1:10000 HRP conjugated in TBST, 1h, RT

Tertiary Incubation

N/A

Detection

ECL, Chemiluminescence

Results Summary

Antibody showed perfect binding to protein.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High sensitivity

The Bad

Relative high background signal

The Bottom Line

Excellent

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