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Alexa Fluor® 700 anti-mouse CD3 Antibody
100215
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I've used this antibody in a number of flow cytometry assays in mouse samples. I've always produced good results with this antbody. The only issue I have with it is when doing fix-perm assays this antibody tends to give a high background staining, but it is still possible to distinguish positive and negative populations. In unfixed samples the staining is always excellent with very little background.
Flow Cytometry
Mouse blood, spleen, lung, bone marrow
1:100 dilution, 30 min @ 4C
Fc block (Anti CD16/CD32)
None
Flow cytometer
In most situations this antibody works very well for flow.
N/A
Strong staining in unfixed samples
High background in fixed samples
Good antibody