Reliable CD4 Antibody For Flow Cytometry

May 29, 2018

BlueskyReddit
GIVI
The Gladstone Institutes
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV650 Mouse Anti-Human CD4

Catalog Number:

563875

My lab investigates markers for detecting the latent HIV reservoir. Specifically, we look at tissue resident memory CD4 T-cells. This antibody shown to reliably gate on the CD4+ populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Colonoscopies

Primary Incubation

1:300 at 4C for 30 min

Blocking Agent

1:1000 FC block at 4C for 30 min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Results clearly separate the CD4 population from the rest of the CD3+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Great and provides consistent results

The Bad

N/A

The Bottom Line

A great product for important sorts/experiments!

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