Millipore
MAP2
AB5622
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The aim of the research was to understand if Pur- alpha prevented mislocalization of ALS associated FUS protein in neurons. For this we used MAP2 as a neuronal marker.
Immunofluorescence
Rat motor neurons
1:500
10% horse serum with 2% BSA
None
Confocal microscope
Cells were labeled with anti-MAP2 antibody (1:500, Millipore) and Alexa Fluor 647 goat anti-rabbit secondary antibody (1:500, Life Technologies). Z stacks of images were captured sequentially to eliminate bleed-through of the fluorophores. Loss of MAP-2 fluorescence was taken as a measure of cell death.
26728149
N/A
Easy to optimize and use
Effective neuronal marker