Excellent CD8a Antibody

June 01, 2018

BlueskyReddit
Immunology
Gladstone Institutes
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 700 Rat Anti-Mouse CD8a

Catalog Number:

557959

We are interested in CD8+ T cells that lack key signaling proteins. This antibody is very reliable and provides robust data by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

30 minutes @ 4 degrees, 1:100

Blocking Agent

Fc block 30 min @ 4 degrees, 1:1000

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Robust expression shown with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable and gives robust data

The Bad

N/A

The Bottom Line

Perfect antibody for flow cytometry

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