Protein Precipitation Solution

Albert Einstein College of Medicine
Pathology
PhD Candidate

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Company:

Qiagen

Product Name:

Protein Precipitation Solution

Catalog Number:

158910

The goal of our lab is to understand the effect of HIV infection on various aspects of the immune response both in vitro and in vivo. To study these phenomena in vivo, various transgenic mouse lines are utilized including those transgenic for various human and viral genes that require ongoing genotyping efforts. The extraction of adequate yields on quality DNA requires reliable extraction methods. The Qiagen Protein Precipitation Solution has proven to be highly efficient and reliable at removing protein contaminants from DNA extracts from mouse tails for our genotyping.

Experimental Design and Results Summary

Application

DNA extraction

Starting Material

Mouse tail

Protocol Overview

Perform proteinase K digestion followed by cell lysis. Add protein precipitation solution and proceed with DNA extraction using isopropanol.

Tips

Adjust the volume depending on the size of your initial tissue sample

Results Summary

DNA extracts with little protein contamination

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Removes proteins from tissue samples for DNA extractions

The Bad

None

The Bottom Line

Great reagent for removing proteins from tissue samples for DNA extractions.

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